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The ELF Preparative Electrophoresis System for Size-Based Proteome Fractionation

Sage Science

The ELF Preparative Electrophoresis System for Size-Based Proteome Fractionation

15 Oct 2015

Although the resolution of LS-MS systems is improving every year, size fractionation of complex samples prior to MS analysis is still very useful for many proteomic studies. Despite the promise of automated 2D LC systems, SDS PAGE remains a popular, but laborious, pre-fractionation method for MS. This poster demonstrates an automated preparative electrophoresis system (the ELF system) to fractionate complex protein samples into 12 contiguous size fractions. The system uses a two-dimensional process: first, proteins are electrophoresed through an SDS agarose gel column to separate the proteins by size, and second, the separated proteins are electrophoresed sideways out of the agarose column into a linear array of membrane-bounded elution modules that are positioned alongside the separation gel column. The fractionated samples are recovered in SDS buffer, and can be processed for LC-MS using commercial detergent removal spin columns and standard trypsin digestion methods.

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SageELF Sample Fractionator for DNA and Protein

Sage Science

The SageELF is an automated, whole-sample fractionation system for NGS sample prep. SageELF, a whole-sample fractionation tool, is now shipping from Sage Science, developer of the Pippin automated DNA size selection system. The SageELF (short for Electrophoretic Lateral Fractionator) generates 12 contiguous fractions from a whole DNA sample, allowing scientists to generate libraries with multiple insert sizes from the same sample, which has applications in genome sequencing, structural variant detection, and identification of rare splice variants. This sample prep instrument will also be especially useful for preserving precious samples. Users simply load their sample, start the run, and pipette out the fractions later. Unlike in-gel digestion, in which some DNA fragments never leave the gel, the whole DNA sample is electrophoresed into the elution modules.Sage Science targets labor-intensive steps in the sample prep process where automation can offer improved precision and reproducibility. Precise size selection is critical for optimizing sequencing efficiency, improving genome assemblies, and reducing NGS project costs.Features: Make libraries with multiple insert sizes from the same sample Ideal for rare or precious samples Automated for precise, reproducible results Applications: Genome sequencing Structural variant detection Rare splice variant detection

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Size SelectionProteomicsProteomics refers to the analysis of proteins through a variety of different experiments in order to understand structure and functionality. Typical techniques include protein purification and mass spectrometry. Digestion

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