The DeNovix SmartPath® and High Absorbance

This application note provides some background on the relationship between the terms transmittance and absorbance as well as describe the Beer-Lambert law. This information will then be used to explain how the DS-11 enables measurement of samples with ultra high concentrations. The DeNovix® DS-11 spectrophotometer utilizes proprietary SmartPath® Technology in conjunction with an innovative microvolume design (patent pending) to enable measurements of samples with absorbance values as high as 500 AU (at a 1cm equivalent pathlength). This means that bovine serum albumin (BSA) samples up to 750 mg/ml and dsDNA samples up to 25000 ng/μl can be accurately and reproducibly quantified using the DS-11.