ResourceLife Sciences
Targeted bisulfite sequencing: An efficient, streamlined approach to maximize throughput and minimize cost
2 Dec 2020In this application note, QIAGEN present a streamlined workflow combining optimal bisulfite treatment and targeted bisulfite sequencing. This method utilizes single-primer extension (SPE) and unique molecular identifier (UMI) barcoding technology. This enables highly efficient, flexible and scalable targeted analysis of DNA methylation from genomic DNA (gDNA), formalin-fixed paraffin-embedded (FFPE) and circulating cell-free DNA (ccfDNA) samples.
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Epigenetic AnalysisEpigenetic analysis products are used to study heritable phenotype changes that do not alter the DNA sequence, such as DNA modifications affecting gene activity. Determine whether genes have been methylated with DNA methylation quantification kits or methylation microarrays. Assess epigenetic regulation with histone modification kits or ChIP-on-chip microarrays. Kits are also available for bisulfite conversion and amplification of your samples, as well as epigenomic library preparation kits for next-generation sequencing (NGS). Find the best epigenetic products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA MethylationMethylationSequencingSequencing refers to determining the order of nucleotides in a DNA or RNA molecule. This process is essential in genomics, diagnostics, and evolutionary biology. Techniques like next-generation sequencing (NGS) and Sanger sequencing provide high-throughput and precise data for analyzing genomes and transcriptomes. Browse our peer-reviewed product directory to find the best sequencing tools and reagents, compare products, check reviews, and get pricing directly from manufacturers.