ResourceLife Sciences
SuperNova v428: New bright polymer dye for flow cytometry applications
21 Apr 2021SuperNova v428 is a new violet laser (405 nm)-excitable water-soluble polymer dye which shows exceptional brightness. This is attributed to its high extinction coefficient (~3 million Lmol-1 cm-1) and fluorescence quantum yield (~0.6) in phosphate buffer. Excitation maximum of this polymer is measured as 414 nm and its emission maximum is at 428 nm (Figure 1) which can be detected using a 450/50 bandpass filter or equivalent in a flow cytometer.
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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.