ResourceLife Sciences
Superior High-throughput Genotyping with KlearKall Master mix and BHQplus probes
4 Oct 2015High-throughput genotyping of SNPs (single nucleotide polymorphisms) is used to analyze large numbers of human samples in genome-wide association studies and for validating any disease-linked genetic variants found. Analysis of large numbers of samples accurately and efficiently is fundamental to this process. In agriculture, using SNPs as desirable trait markers is a powerful tool to accelerate breeding programs for crop improvement. Crude DNA extracts are commonly used for this as they are rapid and cost effective to prepare. However, crude extracts produce poor quality DNA and carry debris and endogenous PCR inhibitors. This application note demonstrates the use of an optimized PCR mix high-quality, high-throughput endpoint genotyping with hydrolysis probes in low (1 μL) reaction volumes.
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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.SNPs TechnologySingle nucleotide polymorphisms (SNPs) are individual base variations in a DNA sequence. SNPs are used in research to study predispositions to disease and drug discovery. Products for studying single nucleotide polymorphisms include SNP arrays and detection systems to detect polymorphisms, SNP typing systems for genotyping DNA, and PCR to amplify specific SNPs.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AgricultureAgriculture research focuses on improving food production, crop management, and sustainability. This includes plant breeding, pest control, and soil health studies. Explore agriculture research tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.PCR PurificationSNP Genotyping