ResourceSeparations

Successful Transfer of Size-Exclusion Separations between HPLC and UPLC

24 Nov 2014

Waters’ recent development of HPLC compatible 200Å and 450Å, 3.5 µm particles based on the same diol-coated BEH-particle chemistry as that used in ACQUITY UPLC Protein BEH SEC Columns provides the capability to readily transfer between SE-UPLC and SE-HPLC for the first time. This application note details and provides examples of the methodology that can successfully employed for this method transfer.

ACQUITY UPLC H-Class PLUS Bio System

Waters

The ACQUITY UPLC H-Class PLUS Bio System delivers the benefits of UPLC's resolution, sensitivity, and throughput in a system purpose-built for the analysis of proteins, peptides, nucleic acids, and glycans.

(7)

ACQUITY UPLC BEH Columns

Waters

In 2004, Waters revolutionized liquid chromatography with the introduction of the ACQUITY UPLC System. At the heart of the system was Waters second generation hybrid, the Ethylene Bridged Hybrid (BEH) particle. In 2005, BEH Technology preparative particle sizes (2.5, 3.5, 5 and 10 μm) with the introduction of XBridge between HPLC and UPLC Technology platforms. BEH Technology particles offer: Excellent peak shape and efficiency for basic analytes A rational array of chromatographic selectivity Improvements in chemical stability at mobile phase extremes, particularly at elevated pH

(41)

Links

Tags

Successful Transfer of Size-Exclusion Separations between HPLC and UPLC