ResourceSpectroscopy

Streamlining MALDI workflows in the microbiology lab

20 Feb 2024

In this application note, Bruker highlights the benefits of disposable target plates for cost-effective and convenient microorganism identification workflows. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is praised for its ability to overcome the limitations of classical biochemical methods, offering rapid and specific results. MALDI-TOF MS utilizes proteomic fingerprinting to identify bacteria, yeast, and fungi species. The technique's speed, accuracy, and simplicity in sample preparation make it ideal for routine and high-throughput microbial identification. MALDI-TOF MS target plates, typically made of metal, plastic, or ceramic, hold samples for analysis, with disposable plates offering the advantage of immediate use without the need for cleaning and checking between runs.

MALDI Biotyper® sirius System

Bruker Microbiology & Infection Diagnostics

The MALDI Biotyper® employs MALDI-TOF mass spectrometry for microbial identification, facilitating unbiased identification of microorganisms within a few minutes down to the species level, starting from culture. It boasts various features, such as ease of use, rapidity, robustness, high throughput, cost-effectiveness, and efficiency, making it a must-have for microbiology laboratories.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microbiology ConsumablesMicrobial DetectionMicrobial detection is crucial for identifying and quantifying bacteria, viruses, fungi, and other microorganisms. This process is essential for food safety, environmental monitoring, clinical diagnostics, and pharmaceutical manufacturing. Explore the best microbial detection tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.
Streamlining MALDI workflows in the microbiology lab