ResourceLife Sciences
Streamlined Spheroid-Based Drug Discovery Luminescent Assay Using PrimeSurface 384 Well White Plates
10 Apr 2019In this application note, spheroids are generated in PrimeSurface ULA plates and analyzed by luminescent assays for high-throughput screening.
Related products
Request Quote for All Products
PrimeSurface® 3D Culture Spheroid White Plates
S-BioChemiluminescent assays often outperform analogous fluorescent assays due to its’ low background offering a high signal to noise ratio, thus providing higher assay sensitivity. Spheroid tumor cells are typically generated using ULA plates and other 3D cell culturing methods, however transferring them without damaging the spheroids to a 2D white plate for luminescent assay screening can be challenging. Using PrimeSurface® 96 and 384 well ULA white plates, cancer spheroid cells can be formed and analyzed in the same multiwell plate for luminescence assays.
Links
Tags
Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.SpheroidsSpheroids are clusters of cells that have been grown in 3D culture to be used as <i>in vitro</i> model systems. These 3D microtissues can be used for toxicology testing, DMPK studies and many other applications involving cell analysis.LuminescenceLuminescence techniques detect and measure light emitted by substances during chemical reactions or biological processes. Widely used in assays for diagnostics, environmental monitoring, and drug screening, luminescence-based methods offer high sensitivity. Explore leading systems and products in our directory.Drug Discovery & Development Screening