Streamline PCR and qPCR workflows with automated liquid handling

28 Sept 2022
Streamline PCR and qPCR workflows with automated liquid handling eBook preview

qPCR is a very versatile technique with many applications, however, it is also very sensitive and therefore requires accuracy and precision in liquid handling in order to reduce technical errors, cross-contamination, and yield reliable data.

In this free eBook, we present a number of high-throughput sample preparation methods for synthetic and molecular biology applications, and explore how automation in liquid handling can transform the throughput, accuracy, and reliability of qPCR and nucleic acid normalization in different laboratory workflows.

Download your free copy of the eBook to discover solutions for:

  • Ensuring sample integrity in qPCR
  • Optimizing qPCR master mix dispensing
  • RNA normalization for qPCR
  • DNA normalization for qPCR
  • Streamlining plant RNA extraction for RT-qPCR
  • Standardizing RT-qPCR workflows for SARS-CoV-2

Gilson PLATEMASTER 20uL

Gilson, Inc.

PLATEMASTER® is an easy-to-use, accurate solution for high-throughput manual pipetting of 96- and 384-well microplates. Two models are available: PLATEMASTER P20 for pipetting volumes from 0.5−20 µL and PLATEMASTER P220 for pipetting volumes from 2−220 µL.  Increase the Productivity of Your Workflows The PLATEMASTER’s 96-channel design greatly reduces the number of pipetting steps necessary to fill a microplate when compared to using regular manual multichannel pipettes. When using PLATEMASTER, the time it takes to fill 96-well plates is significantly reduced to approximately 10-20 seconds or less, and 384-wells can typically be filled in less than a minute using only four pipetting steps. Increased Accuracy, Precision, and Reproducibility Using PLATEMASTER for higher throughput applications reduces the risk of human error. In a single motion, accuracy and precision levels can equal that of an 8- or 12-channel pipettes, while avoiding the risk of skipping or repeating wells. Additionally, all enzymatic reactions and cell-based applications across all wells can be started and stopped at the same time. From 0.5 µL to 220 µL pipetting volumes, expect improved well-to-well and plate-to-plate reproducibility. Versatile and Easy to Use PLATEMASTER is as intuitive as a PIPETMAN® and does not require programming, long training, or power for use. Through a variety of accessories, such as adapters for loading 384-well microplates, reagent reservoirs, or sample heater blocks, PLATEMASTER can be fully adapted to a variety of application needs. Its compact and portable design easily integrates into the lab, allowing it to fit on most benchtops, under hoods, or in cold rooms.

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Gilson PIPETMAX

Gilson, Inc.

PIPETMAX® is an automated pipetting solution for the efficient processing of high-throughput biological assays. It will help you to improve on the accuracy, reproducibility, and consistency among all samples processed. Unlike traditional automation platforms, PIPETMAX comes in a benchtop size that easily fits into any lab.

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.Liquid Handling WorkstationLiquid handling workstations automate the process of sample preparation, improving accuracy and efficiency. These systems are essential for high-throughput applications in biological, chemical, and pharmaceutical research, reducing human error and increasing reproducibility. Workstations come equipped with robotic arms, pipetting tools, and other accessories to streamline lab workflows. Explore liquid handling workstations in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.PCR AutomationPCR automation describes the process of using a thermocycler to complete the polymerase chain reaction (PCR) process quickly and efficiently.Sample ProcessingSample processing is required to prepare samples prior to analysis. Technologies used for sample processing include homogenizers, grinders, mills and mixers.RT qPCRReal-time quantitative PCR (RT-qPCR) is a technique used to measure gene expression and quantify DNA or RNA in real-time. It is essential in genomics, diagnostics, and biotechnology research. Explore RT-qPCR systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Real-Time PCRReal-time PCR (RT-PCR) is a technique used to amplify and simultaneously quantify nucleic acids in real time. It is a key tool in gene expression analysis, pathogen detection, and diagnostics. RT-PCR allows for the detection of specific RNA or DNA sequences with high sensitivity and specificity. Browse our peer-reviewed product directory to find the best Real-Time PCR systems; compare products, check reviews, and get pricing directly from manufacturers.