ResourceLife Sciences
Stem Cell Research in The Fight Against Disease
Stem Cell Research in The Fight Against Disease
9 Jan 2016This application note comes from Binder and looks at the importance of embryonic stem cells in research today. It looks at a group from The Huck Institutes of the Life Sciences at the Pennsylvania State University who specialize in embryonic stem cell research and use equipment from Binder to ensure a sterile environment when culturing the cells.
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Cell Adhesion AssaysCell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for extracellular matrix adhesion, cell migration and sensitivity to inhibitors. Find the best cell adhesion assay equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Data AnalysisData analysis hardware and software is available to make data processing straight-forward yet powerful. Data software can be used for math and stats, technical graphing and image analysis. In addition, software is available for specific data analysis of electrophoresis, densitometry, ELISA and DNA sequencing.IncubatorsLaboratory incubators are used to provide a stable environment to cultivate and store samples in, giving you optimal control of temperature, humidity, oxygen and CO<sub>2</sub> levels. Incubators are necessary equipment for cell culture and microbial research. Control temperature with heated or refrigerated incubators, or the levels of CO<sub>2</sub> and oxygen with CO<sub>2</sub> incubators. Using environmental chambers, create a humid climate for testing samples. Find the best incubators in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Washing EquipmentWashing equipment is more convenient than ever before with automated systems, integrated and multi-application systems available. For instance microplate washers can be programmable, combined with reagents dispensers and used for applications such as ELISA, multiplex assays and PCR clean up.Cellular PathologyCellular Pathology deals with the microscopic analysis of tissue samples and cells. Sample preparation and processing includes fixation, staining, sectioning and slide mounting, using equipment such microtomes and cryostats. In choosing immunohistochemistry and immunocytochemistry kits, consider chromogens, staining method, antibodies, microscopes and imaging.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Evaporators and Freeze DryersCentrifugal evaporation is used for solvent removal from a sample. This is a useful technique for sample concentration or sample drying. Many centrifugal evaporators are modular with a central vacuum pump for generating low pressure. Useful features of evaporators or vacuum ovens include minimal solvent bumping, solvent resistance, computer control, temperature regulation and benchtop design.RegulatoryPharmaceutical regulations impact on all areas of drug development, manufacture and control and supply. Services are provided from the early stages of drug development and clinical trials through to dossier submission, approval and marketing. Regulatory also includes submission of analytical studies from the pharmaceutical product and safety. Fumehoods and Biological Safety CabinetsFume hoods and biological safety cabinets are used to provide personnel, sample and environmental protection by limiting exposure to hazardous materials either by venting or enclosing hazardous sample space. Fume hoods include filtered and ductless fume hoods, general ducted fume hoods, laminar flow cabinets and cabinets for powder weighing. There are three classes of biological safety cabinet, dependent on the level of protection provided. Class I provide personnel and environmental protection, class II provide product, personnel and environmental protection. Class III provide the highest level of protection and are designed for use with high-risk biological agents. Other ventilated workspaces for the laboratory include glove boxes, glove bags, anaerobic and hypoxic workstations. Find the best fume hoods and biological safety cabinets in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Stem Cell ResearchStem cells have the ability to produce identical daughter cells as well as progeny, which commit and differentiate. Stem cell research has exciting prospects of biomedical applications in cellular disease modeling, pharmaceutical screening, and regenerative medicine. Embryonic Stem CellsContamination PreventionContamination prevention aims to control and minimize the risk of contamination during experiments. Biological safety cabinets and sterilization equipment are examples of technologies used to prevent contamination.Sterilization