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Static Light Scattering Technologies for GPC/SEC Explained

4 Jul 2013

The aim of this guide is to provide the reader with a clear understanding of the different technological approaches used to measure molecular weight by static light scattering in a GPC/SEC experiment. It will explain and differentiate between the techniques and technologies used in SLS, MALS, RALS, and LALS. It assumes no prior knowledge of the light scattering theory or instrumentation and should be ideal for those new to light scattering and those looking to increase their knowledge in the area. The guide covers an introduction to the theory and background of molecular weight measurements by static light scattering. It is hoped that the information contained in here will help users to make an informed decision about the most appropriate light scattering technology to use.

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Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Dynamic Light ScatteringDynamic light scattering is used to measure the size distribution of particles in suspension by analyzing the way light scatters off them. It is commonly used in materials science, nanotechnology, and protein characterization. Browse our peer-reviewed product directory to find the best dynamic light scattering systems, compare products, check reviews, and get pricing directly from manufacturers.