ResourceSeparations
SPE Extraction and LC /MS /MS Analysis of a Peptide from Human Serum
21 May 2015This application note describes an ultra-sensitive extraction method to isolate a peptide, Goserelin, from serum using Strata-X-CW μElution 96-well SPE plates followed by LC/MS/ MS analysis using an Aeris WIDEPORE 3.6 μm XB-C18 HPLC column. By using the μElution SPE format, we were able to skip the dry down step which saved at least 30 minutes and also helped to avoid loss of the peptide. Goserelin was analyzed by LC/MS/MS in under 3 minutes and was accurately quantified at 5 ng/mL.
Related products
Request Quote for All Products
Strata® Solid Phase Extraction
Phenomenex IncAvailable retention mechanisms include reversed phase, ion exchange, normal phase and mixed mode
Links
Tags
Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Human SerumHuman serum is found in blood plasma. The protein is used in a variety of applications including tissue engineering, transplantation and cell therapy.