Spatial proteomic analysis of immune cells in Alzheimer’s Disease human brain using multiplexed imaging and AI-assisted phenotyping

Explore how Cell DIVE multiplexed imaging creates an intricate cellular map of Alzheimer's disease (AD) brain tissue. By visualizing diverse cellular phenotypes—including astrocytes, microglia, AD-specific markers, and various immune cells—we delve into the cellular complexity of AD pathology. AI-powered tools within Aivia are employed to characterize unique neuro-immune interactions, enhancing our understanding of the immune landscape in Alzheimer's.

A focal point of the study is the spatial analysis of β-amyloid plaque neighborhoods, where astrocytes, microglia, and immune cells localize and exhibit specialized expression profiles. By comparing immune cell activity in regions proximal and distal to plaques, we identify distinct spatial immune dynamics within AD brain tissue. The findings highlight how multiplexed imaging, combined with AI-driven analysis, provides critical insights into cellular interplay in AD, offering potential pathways for targeted therapeutic interventions.