Spatial profiling of immune cell gene signatures in the tumor microenvironment

19 Jan 2022

Complex tissues with high cellular heterogeneity require single-cell technologies both at the transcriptomic and spatial level to fully interrogate the cell types within them. This application note demonstrates the capabilities of a multiplexed in situ transcriptomic approach for the spatial mapping of target genes in highly complex and heterogenous FFPE tumor tissues using the RNAscope HiPlex v2 assay.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.GenomicsGenomics is the study of genomes, focusing on the sequencing, analysis, and interpretation of genetic material. It is key in understanding genetic diseases, evolutionary biology, and personalized medicine. Techniques like next-generation sequencing (NGS) are commonly used in genomics research. Browse our peer-reviewed product directory to find the best genomics tools, compare products, check reviews, and get pricing directly from manufacturers.TranscriptomicsMultiplexingMultiplexing refers to the ability to measure multiple targets or analytes simultaneously in a single experiment. This technique is valuable for high-throughput screening, diagnostics, and complex assays, as it increases efficiency and data quality. Browse our peer-reviewed product directory to find the best multiplexing products, compare tools, check reviews, and get pricing directly from manufacturers.
Spatial profiling of immune cell gene signatures in the tumor microenvironment