Solving the Aggregation Problem of Human Embryonic Kidney 293 Cells Using the New Brunswick™ S41i CO<sub>2</sub> Incubator Shaker

25 Aug 2015

This application note shows a successful example of the adaptation of a human recombinant protein-expressing HEK293 cell line from serum-supplemented attachment culture to serum-free single cell suspension culture using the New Brunswick S41i CO2 incubator shaker. This adapted HEK293 cell line behaves like a typical aggregationless suspension cell line.

New Brunswick S41i Incubator Shaker

Eppendorf

For over a decade New Brunswick CO2 incubators have provided researchers with a reliable, easy-to-use system for culturing cells. Continuing this tradition is the New Brunswick S41i CO2 Incubator Shaker, designed specifically for non-adherent cell culture applications. The S41i combines precise temperature and CO2 control with the best laboratory shaker available. The result: high cell yields and viability. Like all New Brunswick CO2 incubators, the S41i is loaded with advanced features. A unique six-sided direct-heating profile provides gentle convection circulation of the chamber atmosphere for exceptionally uniform temperature control. But what makes the S41i really special is the built-in New Brunswick shaker. Combining our advanced CO2 incubator and shaker technologies creates a highly stable environment for reliable cell growth. When it comes to high cell yields and viability, as well as overall reliability, you won’t find a better solution. Features: Fanless design eliminates a common source of repeated contamination and expensive HEPA filters Sealed inner/outer doors and advanced PI control maintain temperature accuracy and uniformity while minimizing costly gas consumption High temperature disinfection (HTD) protects against bacterial contamination Unique InfraRed (IR) CO2 sensor offers specific measurement and accurate control of CO2 levels 25 mm access port for adding instrumentation or additional probes USB port for communication and external instrument logging Can be used on the floor, under counter or double stacked to save space; ideally sized for a cell culture lab Includes removable stainless steel humidity pans Optional BioCommand® SFI software for remote and historical data logging and report generation

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.IncubatorsLaboratory incubators are used to provide a stable environment to cultivate and store samples in, giving you optimal control of temperature, humidity, oxygen and CO<sub>2</sub> levels. Incubators are necessary equipment for cell culture and microbial research. Control temperature with heated or refrigerated incubators, or the levels of CO<sub>2</sub> and oxygen with CO<sub>2</sub> incubators. Using environmental chambers, create a humid climate for testing samples. Find the best incubators in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Shakers, Stirrers and MixersShakers, stirrers and mixers are used for mixing liquids and solids for laboratory applications. There is a wide range of laboratory mixing equipment available, including magnetic stirrers, orbital shakers, linear shakers, microplate shakers, 3D gyratory rockers, rotators & vortexers. Additional platforms and clamps can also be attached to shakers to increase capacity. Find the best shakers, stirrers and mixers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.AggregationHEK CellsCell GrowthCell growth refers to the process by which cells increase in size and number. It is essential for tissue development, wound healing, and cancer research. Understanding and controlling cell growth is a fundamental part of cell biology and regenerative medicine. Browse our peer-reviewed product directory to find the best tools for studying cell growth, compare products, check reviews, and get pricing directly from manufacturers.
Solving the Aggregation Problem of Human Embryonic Kidney 293 Cells Using the New Brunswick™ S41i CO<sub>2</sub> Incubator Shaker