ResourceLife Sciences
Solving the Aggregation Problem of Human Embryonic Kidney 293 Cells Using the New Brunswick™ S41i CO<sub>2</sub> Incubator Shaker
25 Aug 2015This application note shows a successful example of the adaptation of a human recombinant protein-expressing HEK293 cell line from serum-supplemented attachment culture to serum-free single cell suspension culture using the New Brunswick S41i CO2 incubator shaker. This adapted HEK293 cell line behaves like a typical aggregationless suspension cell line.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.IncubatorsLaboratory incubators are used to provide a stable environment to cultivate and store samples in, giving you optimal control of temperature, humidity, oxygen and CO<sub>2</sub> levels. Incubators are necessary equipment for cell culture and microbial research. Control temperature with heated or refrigerated incubators, or the levels of CO<sub>2</sub> and oxygen with CO<sub>2</sub> incubators. Using environmental chambers, create a humid climate for testing samples. Find the best incubators in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Shakers, Stirrers and MixersShakers, stirrers and mixers are used for mixing liquids and solids for laboratory applications. There is a wide range of laboratory mixing equipment available, including magnetic stirrers, orbital shakers, linear shakers, microplate shakers, 3D gyratory rockers, rotators & vortexers. Additional platforms and clamps can also be attached to shakers to increase capacity. Find the best shakers, stirrers and mixers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.AggregationHEK CellsCell GrowthCell growth refers to the process by which cells increase in size and number. It is essential for tissue development, wound healing, and cancer research. Understanding and controlling cell growth is a fundamental part of cell biology and regenerative medicine. Browse our peer-reviewed product directory to find the best tools for studying cell growth, compare products, check reviews, and get pricing directly from manufacturers.
