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Solutions for Mass Spectrometric Detection from Merck Millipore

Merck KGaA, Darmstadt, Germany
6 Nov 2014

Mass spectrometry (MS) is nowadays an established routine detection method that can be coupled to various techniques in the laboratory. In contrast to more simple detectors such as ultraviolet-visible (UV-Vis) or fluorescence, MS generates data about molecular masses and detailed structural parameters and is by far more sensitive in the wide majority of applications. Many sophisticated as well as affordable systems are available, covering various fields of use such as quantification and qualification experiments, and a number of these systems are described in this compendium.

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LiChrosolv® Hypergrade Solvents for LC-MS

Merck KGaA, Darmstadt, Germany

High purity solvents for LC-MS and trace analysis with UV and fluorescence detection: 0.2 µm stainless steel filtered – perfect for use in UPLC and UHPLC. Very high UV-transmittance. Excellent baseline stability in gradient elution. Very low total ionic current (TIC) in LC-MS. Low level of ionic background and ion suppression. High ionization efficiency in all ionization methods (electrospray ionization/atmospheric pressure chemical ionization (ESI/APCI) positive and negative mode). Applications: Protein profiling. Pesticide Analysis. Polycyclic aromatic hydrocarbons (PAHs). Proteomics LC-MS. LC-MS routine analysis. Lichrosolv® hypergrade solvents enable analysis in the low ppb trace range and can be used for both the isocratic separation of 6 PAHs according to the German DIN method and the gradient separation of 16 PAHs according to the methods EPA 610 (analysis of drinking water) and EPA 550 + benzo(e) pyrene + perylene (analysis of waste water).

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Purospher® STAR HPLC and UHPLC columns

Merck KGaA, Darmstadt, Germany

The base silica of Purospher® STAR columns is 99.999 % pure. Furthermore, meticulous care is given to quality control over all aspects of silica structure and chemistry. The excellent converge of the surface and the purity of the material ensures excellent peak symmetry for acidic, basic and even for chelating compounds. Accurate results with excellent peak shape for all types of analytes Outstanding resolution due to high separation efficiency Proven reliability and reproducibility from run to run and batch to batch Universal compatibility with best all-round performance according to Tanaka Maximum flexibility in method development and choice of mobile phase pH stability from pH 1.5 – 10.5 Suitable for up to 100 % aqueous mobile phases Highest sensitivity and suitability for LC-MS applications

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SeQuant® ZIC®-cHILIC for HPLC and LC-MS separation of polar hydrophilic compounds

Merck KGaA, Darmstadt, Germany

This new zwitterionic stationary phase with phosphorylcholine functional group provides you with complementary selectivity for easier method development for substances that have been difficult to separate by previous types of HPLC columns operated in reversed-phase or HILIC mode. The selectivity features of SeQuant® ZIC®-cHILIC are especially beneficial for negatively charged polar compounds such as nucleotides and organic acids, but do also provide advantageous separations of positively charged hydrophilic molecules; for example aminoglycosides and cations.   The column is also very suitable for zwitterionic and neutral polar hydrophilic substances. High-performance HILIC column Complementary selectivity for polar hydrophilic compounds Maximum LC-MS compatibility with minimized column bleed Excellent reproducibility and robustness Very high stability (more than 1600 injections during 270 hours of continuous use without showing any signs of change in retention)

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.GC-MS GC-MS (gas chromatography-mass spectrometry) instruments and equipment are used to separate, quantify and identify mixtures of small and volatile compounds, such as polycyclic aromatics, fatty acids and alcohols. Often used in drug detection, forensic investigation and environmental analysis for pesticides and contaminants, GC-MS is a powerful addition to your lab’s analytical capabilities. GC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzers can give greater sensitivity and resolution to your analysis. Find the best GC-MS instruments and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Method DevelopmentMethod development is the process of creating and optimizing experimental techniques and protocols to achieve reliable, reproducible results. This is essential in various fields, including pharmaceuticals, environmental science, and diagnostics. Browse our peer-reviewed product directory to find the best method development solutions, compare products, check reviews, and get pricing directly from manufacturers.

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Solutions for Mass Spectrometric Detection from Merck Millipore