Solution for Effective Removal of Cell Debris and Subsequent Purification of Biomolecules

15 Mar 2013

This technical note by Pall Life Sciences presents a solution for the most problematic step in the purification of biomolecules which is the clarification of the sample once the cells have been lysed. In the past, centrifugation was the primary method used to sediment cellular debris, this method has several limitations. However, Pall Life Sciences present a lysate sample preparation procedure using the AcroPrep™ Advance filter plate for lysate clearance that effectively removes unwanted cellular debris from samples. The filter plate provides maximum biomolecule recovery and effectively removes cellular debris from samples prior to purification.

AcroPrep™ Advance Filter Plates for Lysate Clearance

Pall Life Sciences - Laboratory, Food, Beverage

The AcroPrep™ Advance filter plates for lysate clearance effectively remove cellular debris quickly and allow for the use of additional wash steps to maximize sample recovery. Filtration can be accomplished by either vacuum manifold or centrifugation, ultimately maximizing your choice in protocols. The purification of biomolecules (DNA, RNA and proteins) from prokaryotic and eukaryotic cell lines can be problematic. The AcroPrep™ Advance filters plates for lysate clearance provide a prefilter for relatively quick and easy clarification of samples: in addition, the new well geometry ensures faster flow rates and reduced hold up volumes for efficient recovery of biomolecules.Features: Integrated prefilter yields consistent filtration of samples with high levels of gross particulate New well geometry results in faster, more uniform filtration rates across the plate with reduced hold-up volume Manufactured with biologically inert materials that allow clarification of most types of lysates without loss of the target molecule Applications: Removal of Bacterial debris prior to plasmid purification Removal of bacterial and cellular debris prior to protein purification Clearance of gross particulates

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Solution for Effective Removal of Cell Debris and Subsequent Purification of Biomolecules