ResourceSeparations

Solid Phase Extraction Method for Analysis of THC, THC-OH, and THC-COOH in Whole Blood

16 May 2013

This application note describes a method that is used for the extraction of THC, THC-OH, and THC-COOH in whole blood. LC/MS/MS instrumentation is used for analysis with a linear range of 1 ng/mL to 100 ng/mL with average recoveries of individual cannabinoids found to be greater than 95%.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.THC in bloodBlood AnalysisThe analysis of blood is vital for many areas of life sciences and forensic investigations. Blood samples can be tested for a number of different reasons such as diagnosis, glucose levels, cholesterol and drug testing.Drugs of AbuseA drug of abuse is a drug that has been taken for non-medical purposes. Such drugs can have mind-altering effects, with some causing dependence and addiction.