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SmartFlare™ RNA Detection Probes: Principles, Protocols and Troubleshooting

Merck
29 Mar 2015

RNA detection traditionally requires transfection, laborious sample prep, RNA amplification and/or detection based on standard curves. In contrast, SmartFlare™ RNA Detection Probes are endocytosed by live cells using existing cellular endocytosis machinery. This technical note describes how to use SmartFlare probes and how to fix any problems you may experience.

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SmartFlare RNA Detection Probes

Merck

SmartFlare Probes work by recognizing specific native RNA sequences within living cells, therefore there are many possible sequences that you could design to recognize your target of interest. Instead of lysed cells, switch to live cells. And while you’re at it, eliminate sample preparation and transfection steps all together. Live cell RNA detection is now possible, in a single incubation step using inert nanoparticle technology to specifically detect native RNA. And when you’re done, the probes exit the cells allowing you to perform downstream analyses with the same, unperturbed cells. Make the smart change!SmartFlare RNA Detection Probes are fueled by the NanoFlare technology developed by renowned chemist Dr. Chad Mirkin.What's Smart? Detect RNA expression in live cells for real time, physiologically relevant data Eliminate laborious, costly sample preparation Easy analysis on the fluorescent detection platform of your choice Nanoparticle based technology that allows cells to be used for downstream assays

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NanoparticlesNanoparticles are between 1-100nm in size. Nanoparticles can be used for a wide variety of applications including biomedical, catalysis and electronics.

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SmartFlare™ RNA Detection Probes: Principles, Protocols and Troubleshooting