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Size Exclusion Chromatography Method Optimization using the European Pharmacopoeia for Insulin Fibrils

Phenomenex Inc

Size Exclusion Chromatography Method Optimization using the European Pharmacopoeia for Insulin Fibrils

4 Apr 2016

Insulin is a peptide hormone which plays a central role in metabolism. In its monomer form, insulin can partially misfold, causing a cascade of non-native cross assembly of beta-pleated sheets or amyloid-like fibrils. A common method for analyzing insulin HMWP is size exclusion chromatography (SEC). However, the method requires a lengthy 35 minute run time which is time prohibitive for any laboratory with many samples to run and data points to generate. This application shows the method transfer of the Ph. Eur. method from a traditional, 10µm hydrophilic silica 300 x 7.8mm column to a high performance, 1.8µm hydrophilic silica 150 x 4.6mm column.

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Yarra Ultra-High Efficiency Aqueous GFC/SEC Columns

Phenomenex Inc

Starting with 3 µm ultra-pure silica, Yarra particles are densely bonded with a proprietary surface chemistry. Coupled with tight particle and pore size distribution as well as strict packing and QC specifications, Yarra GFC columns allow for the highest efficiencies and resolution possible. Yarra columns are offered in three phases (SEC-2000, 3000, and 4000), and are ideal for the separation of small to large proteins, as well as biological therapeutics and biosimilars.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Method DevelopmentMethod development is the process of creating and optimizing experimental techniques and protocols to achieve reliable, reproducible results. This is essential in various fields, including pharmaceuticals, environmental science, and diagnostics. Browse our peer-reviewed product directory to find the best method development solutions, compare products, check reviews, and get pricing directly from manufacturers.HormonesPeptidesPeptides are short chains of amino acids that play key roles in biological processes, including signaling, immune responses, and enzyme regulation. Peptide research is critical in drug discovery, diagnostics, and vaccine development. Browse our peer-reviewed product directory to find the best peptides and peptide-related products, compare options, check reviews, and get pricing directly from manufacturers.InsulinMethod Transfer

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