ResourceLife Sciences
Site-Specific Labeling of Antibodies for MicroScale Thermophoresis
27 Jan 2017Site-specific conjugation of a dye to antibodies is useful for producing highly homogeneous material for use in downstream affinity experiments. This application note describes a labeling procedure that combines the selective reduction of antibody interchain disulfides and their labeling with maleimide-based chemistry. Different antibodies conjugated by this approach yield high-quality MST affinity data for interactions with both antigens as well as Fc receptors.
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.LabelingAntibody ArraysConjugationMicroscale ThermophoresisSLASThe Society for Laboratory Automation and Screening (SLAS) is an organization focused on laboratory automation, high-throughput screening, and biotechnology innovation. SLAS promotes scientific advancements through conferences, publications, and industry collaborations.