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Single-molecule localization microscopy - Beyond the coverslip

Bruker Fluorescence Microscopy
18 Jul 2023

Traditional microscopy methods have been used for imaging biological organization but are limited to a lateral resolution of ~200-300 nanometers. Single-molecule localization, a super-resolution microscopy approach, allows for high-resolution imaging of specifically labeled biological samples at a resolution of 20 nanometers laterally. Here, Bruker Fluorescence Microscopy summarizes the presentation given by Lauren Gagnon, Applications Scientist for Bruker’s super-resolution microscopy solutions. Presented here are the advantages and principles of single-molecule localization. Also, the unique benefits of the Vutara top-hat illumination and bi-plane technology, as well as the multiplexing capabilities supported by the microfluidics unit, are discussed. Finally, examples of imaging a variety of sample types and popular applications of single-molecule localization microscopy are presented.

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PlexFlo

Bruker Fluorescence Microscopy

Bruker microfluidics accessory units are designed to allow sequential labeling to be incorporated into localization microscopy protocols with the Vutara VXL single-molecule localization microscope. Bruker offers two units—PlexFlo and PlexFlo96. The units are controlled by Vutara's SRX software and allow flexible configuration of fluidics sequences that are run in conjunction with imaging cycles to allow users to label an unlimited number of probes and image them in a sequential fashio

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Vutara VXL

Bruker Fluorescence Microscopy

The Vutara VXL comprehensive biological workstation for nanoscale biological imaging incorporates Bruker’s industry-leading single-molecule localization microscopy (SMLM) technology in a streamlined system with a compact footprint. The new system enables research on DNA, RNA, and proteins and supports advanced spatial biology research. When combined with Bruker’s unique microscope fluidics unit, Vutara VXL enables multiplexed imaging for targeted, sub-micrometer multiomics. 

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Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrofluidicsMicrofluidics is the science of manipulating small volumes of fluids in micro-sized channels. It is widely used in diagnostics, drug development, and lab-on-a-chip technologies. Microfluidic devices can enable rapid, cost-effective, and high-throughput analysis of biological samples. Browse our peer-reviewed product directory to find the best microfluidic devices, compare products, check reviews, and get pricing directly from manufacturers.MicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.Super Resolution Microscopy

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Single-molecule localization microscopy - Beyond the coverslip