ResourceLife Sciences
Single Cell Sorting for Clonal Cell Line Development
7 Dec 2018CHO cells were prepared in a single-cell suspension using Cell Suspension Buffer (PBS, 1% BSA, 1mM EDTA). Viable cells were identified by a lack of propidium iodide (PI) staining. Viable cells were gated on scatter to avoid debris. Doublet-discrimination was performed and singlets were selected for sorting. The Cell Suspension Buffer was used as the sheath buffer. Live, debrisfree, single CHO cells were sorted and dispensed into 96-well plates. The number of cells per well were evaluated using a NyONE plate imager where we observed 80% of the wells to have a single cell with 50% outgrowth to form colonies. The gentle sorting of the WOLF results in improved viability, higher outgrowth, and improved workflow efficiency.
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Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell SortingCell sorting isolates specific cell populations based on physical or molecular characteristics, essential in immunology, cancer research, and regenerative medicine. Techniques like flow cytometry improve accuracy and speed. Compare cell sorting instruments and solutions in our product directory.