ResourceLife Sciences
Simultaneous detection of cell surface phenotype and intracellular functions with superior signal resolution
24 Nov 2025This application note outlines the limitations in functional marker detection with
spectral flow cytometry that can be addressed using CyTOF technology. Download your free copy to receive a direct comparison of spectral flow cytometry and CyTOF intracellular detection, as well as case studies demonstrating robust CyTOF analysis of the major type 2 cytokine IL-13 and the immunoregulatory cytokine IL-10 in human cells.
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BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
