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Simultaneous Analysis of Intact Human Insulin and Five Analogs in Human Plasma Using μElution SPE and a CORTECS UPLC Column

Waters

Simultaneous Analysis of Intact Human Insulin and Five Analogs in Human Plasma Using μElution SPE and a CORTECS UPLC Column

3 Nov 2015

Insulin is perhaps one of the best known and earliest peptide therapeutics. Multiple long and fast-acting analogs have also been developed, and a patient may often be prescribed one of each for diabetes control. Quantification of biologics, such as insulins, has historically been performed using ligand binding assays (LBAs) such as ELISAS. LC/MS/MS, however, has certain advantages over LBAs, such as shorter development times, higher accuracy and precision, the ability to multiplex, no cross-reactivity, and the ability to readily distinguish between closely related insulins. This work provides a single, simple method for the simultaneous, direct quantification of intact human insulin and multiple insulin analogs in human plasma using mixed-mode solid-phase extraction (SPE) and a high-efficiency, solid-core particle column that contains a low-level positive surface charge.

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CORTECS® Columns

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Unleash the power of your laboratory's instrumentation. CORTECS® 1.6 µm and 2.7 µm solid-core columns deliver new levels of performance. CORTECS Columns are available in both reversed-phase and HILIC options, giving you the flexibility and power to develop methods for challenging separations even faster. With different particle characteristics and innovative charged surface modifications, CORTECS Columns are suitable for use in a wide variety of applications. CORTECS Columns offer particle sizes ideal for HPLC, UHPLC, and UPLC platforms, assuring a simple, seamless method transfer with consistent results across different particle sizes and column configurations. CORTECS 1.6 µm and 2.7 µm solid-core columns are suitable for use in a wide variety of applications. CORTECS Columns offer particle sizes ideal for HPLC, UHPLC, and UPLC platforms, assuring a simple, seamless method transfer with consistent results across different particle sizes and column configurations.

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Oasis® µElution Plate

Waters

The Oasis® uElution plate format combined with the Oasis chemistries allow elution volumes as low as 25uL, eliminating the time consuming evaporation step, producing very clean extracts with a 5 to 10 fold increase in concentration.The Oasis® family of sample extraction products is designed to simplify and improve your sample preparation by combining the right sorbent chemistry, device format and methodology. Achieve robust, selective, and sensitive solid-phase extraction [SPE] methods without worrying about low recoveries caused by breakthrough, sorbent drying, pH limitations, and undesirable silanol activity.Oasis® SPE sorbents—covered by eight U.S. patents—are unique in their purity, reproducibility, stability, and retention characteristics. Today, they are the most widely used polymeric SPE products in bioanalytical laboratories.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PeptidesPeptides are short chains of amino acids that play key roles in biological processes, including signaling, immune responses, and enzyme regulation. Peptide research is critical in drug discovery, diagnostics, and vaccine development. Browse our peer-reviewed product directory to find the best peptides and peptide-related products, compare options, check reviews, and get pricing directly from manufacturers.Solid Phase Extraction (SPE)Solid phase extraction (SPE) is a sample preparation technique used to separate and concentrate analytes from complex mixtures. It is widely used in environmental, pharmaceutical, and clinical analysis. SPE provides higher sensitivity and selectivity compared to traditional liquid-liquid extraction methods, making it essential for accurate and reproducible results. Explore SPE tools and systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.InsulinHuman PlasmaHuman plasma is the liquid component of blood, which carries blood components - nutrients, hormones, proteins, and waste - throughout the body. Plasma-based studies provide insights into biomarkers, immune responses, and disease mechanisms. Compare plasma handling tools and systems in our peer-reviewed product directory.TherapeuticsTherapeutics are treatments designed to alleviate or cure diseases. These include pharmaceuticals, biologics, and gene therapies, which work by targeting specific disease mechanisms. Advances in personalized medicine and biologics offer new hope for patients with conditions that were previously difficult to treat. Browse our peer-reviewed product directory to find therapeutic solutions for various diseases; compare products, check user reviews, and get pricing directly from manufacturers.

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Simultaneous Analysis of Intact Human Insulin and Five Analogs in Human Plasma Using μElution SPE and a CORTECS UPLC Column