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Simplified Method Development with the Tecan AC Extraction Plate™

Tecan
2 Mar 2015

The ideal sample preparation process for further analysis by techniques such as LC-MSMS should be simple, involve relatively few steps, produce a clean sample free from interfering matrix materials and, to increase analytical sensitivity, concentrate the analyte of interest. This technical note describes how to use the AC Extraction Plate as a method development tool for specific analytes for LC-MSMS analysis, by optimizing the three ‘pipette and shake’ steps.

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AC Extraction Plate™

Tecan

The Tecan AC Extraction Plate* with TICE™ (Tecan Immobilized Coating Extraction) technology revolutionizes your sample preparation routine. The AC Extraction Plate is a 96-well, deep-well microplate, with each well having a proprietary TICE™ coating. TICE is a material with distinct absorptive properties, and this coating efficiently extracts specific small molecule analytes from aqueous solutions, releasing them when in contact with a suitable organic solvent. A simple pipette and shake sequence, with no filtration, centrifugation or solvent evaporation, is all that is required. The AC Extraction Plate is easily integrated into automated processes, making it a perfect match with Tecan’s Freedom EVO® liquid handling platform. AC Extraction Plate™ Features: Cost-effective – Simple pipette and shake workflow, without labor intensive centrifugation, filtration and solvent evaporation steps. Convenient and fast – Just unpack and start, no conditioning or other preparations needed. Highly reproducible – Pipette and shake routines are easily standardized and automated on a liquid handling platform, eliminating random errors and increasing consistency of results. * For research use only – not for clinical diagnostic applications

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Method DevelopmentMethod development is the process of creating and optimizing experimental techniques and protocols to achieve reliable, reproducible results. This is essential in various fields, including pharmaceuticals, environmental science, and diagnostics. Browse our peer-reviewed product directory to find the best method development solutions, compare products, check reviews, and get pricing directly from manufacturers.

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Simplified Method Development with the Tecan AC Extraction Plate™