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Simple Conversion from ELISA to Luminex® Using the AMG™ Activation Kit from Anteo Technologies.

Anteo Technologies
5 Jul 2015

Luminex® MagPlex® Microspheres may be coupled with antibodies specified for use in ELISA using Anteo Technologies’ AMG™ Activation Kit. This kit uses Mix&Go™ technology to strongly couple antibodies to multiplex microspheres such as Luminex® MagPlex® Microspheres. This application note gives the example of coupling two anti-cytokine antibodies to MagPlex Microspheres, followed by a multiplex immunoassay using the Luminex® system with the matched detection antibodies and standards.

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MagPlex® Multiplexing Microspheres

Luminex

xMAP® Technology uses color-coded microspheres to detect up to 500 protien or oligonucleotide analytes from a single aliquot of sample in a single reaction well, enabling you to generate more data while saving sample, time, and reagents. Depending on your targets and the application, there are five different microsphere types to select from to produce the best experimental results. MagPlex® are magnetic carboxylated polystyrene microspheres that have been dyed into 500 spectrally distinct sets or “regions”, allowing them to be individually identified by an xMAP instrument. In the multiplex assay environment, the microspheres act as both the surface for the solution phase assay and the spectral identifier that the instrument detects. The open architecture of xMAP Technology enables users to build custom multiplex assays or select from a broad menu commercially available kits.  MagPlex are the most versatile xMAP microspheres and are: Compatible with both protein and nucleic acid applications Maximum xMAP microsphere regions available: 500 Superparamagnetic, eliminating the need for filter plates and offering superior washing efficiency Ideal for automated high throughput applications Available in two concentrations: 2.5 (10 regions only) & 12.5 (millions/mL) Available in multiple vial sizes: 1 mL, 4 mL, Custom Compatible with all xMAP instruments: FLEXMAP 3D® (500 regions), Luminex® 100/200™ (80 regions), MAGPIX® (50 regions) Additional xMAP microspheres: MagPlex-TAG™, MicroPlex®, MagPlex-Avidin®, SeroMAP™

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Cell Adhesion AssaysCell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for extracellular matrix adhesion, cell migration and sensitivity to inhibitors. Find the best cell adhesion assay equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.Method DevelopmentMethod development is the process of creating and optimizing experimental techniques and protocols to achieve reliable, reproducible results. This is essential in various fields, including pharmaceuticals, environmental science, and diagnostics. Browse our peer-reviewed product directory to find the best method development solutions, compare products, check reviews, and get pricing directly from manufacturers.Antibody ProductionAntibody production is essential for a wide range of applications in biochemistry and immunology. Ensuring high-quality monoclonal and polyclonal antibodies are produced is key. Explore antibody production tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.MultiplexingMultiplexing refers to the ability to measure multiple targets or analytes simultaneously in a single experiment. This technique is valuable for high-throughput screening, diagnostics, and complex assays, as it increases efficiency and data quality. Browse our peer-reviewed product directory to find the best multiplexing products, compare tools, check reviews, and get pricing directly from manufacturers.

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Simple Conversion from ELISA to Luminex® Using the AMG™ Activation Kit from Anteo Technologies.