Simple and Efficient Full-Length cDNA Library Construction

SMART (Switching Mechanism at 5’ End of RNA Template) is a unique technology that allows the efficient incorporation of known sequences at both ends of cDNA during first strand synthesis, without adaptor ligation. The presence of these known sequences is crucial for a number of downstream applications including amplification, RACE, and library construction. While a wide variety of technologies can be employed to take advantage of these known sequences, the simplicity and efficiency of the single-step SMART process permits unparalleled sensitivity and ensures that full-length cDNA is generated and amplified.The entire SMART protocol is performed by one enzymatic reaction, in a single tube. Your precious RNA is subjected to the least possible handling, minimizing the potential degradation risks. The protocol is user friendly and straightforward with no adaptor ligation, no tailing, and no intervening purification steps.One of the greatest advantages of SMART technology is its increased efficiency compared to traditional technologies such as adaptor ligation. Its high efficiency and sensitivity enables you to use a very limited quantity of starting material, such as microdissected tissues, laser-captured cells, biopsy samples, etc. As little as 1-2 ng of total RNA is sufficient for generating a highly representative cDNA pool for different downstream applications.Features: Start with as little as 1-2 ng of total RNA Specific enrichment for full-length cDNA Optimized protocol for retaining true gene representation Simplified protocol without DNase treatment or a separate adaptor-ligation step Applications: Cloning & library construction Probe generation Subtractive hybridization Next-generation sequencing Virtual northerns Template generation for PCR/qPCR