ResourceSeparations

Silia<i>Chrom</i>® Generic Approach for HPLC Analysis: The Modern Silica-Based HPLC Columns; Silia<i>Chrom</i> Series

21 May 2014

This poster demonstrates that it is possible, with SiliaChrom HPLC products, to consider a generic approach for HPLC method development by using just a few HPLC phases. Using only these three phases, we are able to obtain over 90% success in separating most compounds from different matrices.

HPLC Columns - SiliaChrom®

SiliCycle Inc.

With a choice of over 40 different phases (and we continue to develop additional, unique and powerful HPLC sorbents) our SiliaChrom series of HPLC columns surely has the reversed or normal phase you need for your application.Mostly made from a silica-based sorbent, we carefully monitor the manufacturing process, from raw material synthesis to packing, ensuring you that our HPLC columns are of the highest quality.All columns are packed using a consistent proprietary packing process to achieve a uniform and stable bed, for a long lifespan and column-to-column reproducibility.SiliaChrom HPLC Columns Features: Excellent column efficiency. Long lifetime and column-to-column reproducibility. Broad pH range from 0.8 to 12. Compatibility with 100% aqueous and organic mobile phases. High surface coverage presenting no bleeding for LC-MS applications. Guard columns are also available. Column Characteristics: Phase: C18, C8, C4, C1, Silica, Amide, Amine, Cyano, Diol, HILIC, PFP, Phenyl, SCX, SAX Particle Size: 2.2, 2.5, 3, 5, 10 µm Pore Size: 100, 150, 300 Å Length: 5, 10, 20, 30, 50, 100, 150, 200, 250 mm ID: 2.0, 2.1, 3.0, 4.6, 10, 20, 30, 50, 100 mm

(0)

Links

Tags

UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLC Method DevelopmentIt is important to develop and validate HPLC methods in order to identify, quantify or purify compounds of interest in the most efficient way. Effective method development ensures optimization, quality control and cost effectiveness.
Silia<i>Chrom</i>® Generic Approach for HPLC Analysis: The Modern Silica-Based HPLC Columns; Silia<i>Chrom</i> Series