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Separation of water-soluble vitamins on the agilent InfinityLab Poroshell 120 Aq-C18 Column
20 Jan 2023In this application note, Agilent Technologies describes how ten vitamin B compounds plus ascorbic acid and caffeine were analyzed with an Agilent InfinityLab Poroshell 120 Aq-C18 column using a phosphate buffer and acetonitrile gradient — resulting in good resolution and peak shape.
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InfinityLab Poroshell 120 Aq-C18 Columns
Agilent TechnologiesDesigned to withstand 100% aqueous conditions and increased retention for polar molecules, InfinityLab Poroshell 120 Aq-C18 columns are the modern and reliable choice for HPLC and UHPLC separations. Now master your polar molecules, especially under water.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.VitaminsCaffeineHPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.