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Separation of taurine and caffeine in commercial energy drinks with LC-MS

MicroSolv Technology Corp.
25 Mar 2022

In this application note, a method combining solid phase extraction with high performance liquid chromatography–electrospray ionization tandem mass spectrometry is described for the highly sensitive and accurate screening of caffeine and taurine in energy drinks. This method has been successfully applied to screening of caffeine and taurine in commercial Red Bull samples, and it is valuable to ensure the safety of food and beverages.

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Cogent Diamond Hydride™ HPLC Columns

MicroSolv Technology Corp.

Cogent Diamond Hydride HPLC Columns are manufactured with TYPE-C™ silica (silica hydride) and a very small amount of carbon on the surface that adjusts the column hydrophobicity to an important level. This makes these columns have unique separation capabilities for scientists working in LCMS, metabolomics, bioanalytical, amino acids, organic acids, carbohydrates, and very polar small molecules.

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Solid Phase Extraction (SPE)Solid phase extraction (SPE) is a sample preparation technique used to separate and concentrate analytes from complex mixtures. It is widely used in environmental, pharmaceutical, and clinical analysis. SPE provides higher sensitivity and selectivity compared to traditional liquid-liquid extraction methods, making it essential for accurate and reproducible results. Explore SPE tools and systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.CaffeineBeverage AnalysisBeverage analysis involves testing and evaluating the composition, quality, and safety of drinks, from alcoholic beverages to soft drinks. Techniques like chromatography and spectroscopy are commonly used to analyze flavors, nutrients, contaminants, and additives. Browse our peer-reviewed product directory to find the best beverage analysis tools, compare products, check reviews, and get pricing directly from manufacturers.

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