ResourceSeparations
Separation of Proteins and Peptides with Sartobind® Membrane Adsorbers
10 May 2015In this application note, ion exchange chromatography and Sartobind® membrane adsorbers were used to separate protein and peptide mixtures. All samples were prefiltered with a Minisart filter having 0.2 μm or a 0.45 μm pore size.
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Sartobind IEX Membranes
Sartorius GroupSartobind IEX membranes are macroporous cellulose membranes carrying IEX ligands for the separation and purification of proteins. Our membrane adsorbers feature extremely short cycle times and exceptionally high flow rates and throughput. • Macroporous • High flow rate • Simple handling
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Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.PeptidesPeptides are short chains of amino acids that play key roles in biological processes, including signaling, immune responses, and enzyme regulation. Peptide research is critical in drug discovery, diagnostics, and vaccine development. Browse our peer-reviewed product directory to find the best peptides and peptide-related products, compare options, check reviews, and get pricing directly from manufacturers.