ResourceLife Sciences
Separation and characterization of NISTmAb charge variants with CEInfinite on-line iCIEF-MS
15 Oct 2019On-line iCIEF-MS coupling using the CEInfinite Preparative iCIEF instrument system was verified by the congruent charge isomer peaks between the iCIEF separation of the NISTmAb and its input into the MS, as well as the successful identification of the individual peaks. This technique can easily be applied to other mAbs as well as other protein-based biopharmaceuticals with little additional development from the initial iCIEF method.
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Isogen Life ScienceCEInfinite Preparative facilitates charge variant characterization by direct MS-detection and automated fractionation. CEInfinite is a whole column imaging CIEF, which allows fast method development and high throughput compared to traditional CE. The system is known for its robustness and is FDA CFR 21 Part 11 compliant
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Protein QuantificationThe detection and quantification of proteins in a sample is vital across life sciences, pharmaceutical research and clinical diagnostics, and a variety of equipment is available to scientists to simplify the workflow. Proteins of interest can be easily labeled and detected on light-based detection instruments. Immunoassay kits allow you to identify a specific protein of interest and protein detection beads or antibody microarrays allow you to identify multiple specific proteins at once. Protein interactions and enzyme activity can also be monitored with protein-protein interaction assays. Additionally, the biophysical characterization of proteins is made easy with biokinetic analyzers. Find the best protein detection and quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.
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