Selecting the Right Tool for In-Gel and In-Solution Protein Digestion
16 Aug 2015A variety of problems can occur with in-gel and in-solution protein analysis; poor peptide recovery is common in in-gel digestion whereas incomplete digestion is often seen in in-solution digestion. In this application note, a tailored method for in-gel protein digestion is employed using ProteaseMax, a mass spectrometry compatible surfactant, aimed at minimizing missed tryptic cleavages, leading to increased protein identifications, higher accuracy of protein quantitation and better analytical reproducibility.
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ProteaseMAX™ Surfactant, Trypsin Enhancer
Promega Corp.ProteaseMax™ Surfactant is a high-quality reagent for use in protein and peptide sample preparation. ProteaseMax™ Surfactant solubilizes proteins and ensures fast and improved protein digestion with trypsin or chymotrypsin. It is designed to degrade over the course of a digestion reaction, yielding products that are compatible with downstream methods such as mass spectrometry (MS) and liquid chromatography (LC). For in-gel protein digestion, ProteaseMax™ Surfactant also improves the recovery of peptides. ProteaseMax™ Surfactant can be used with existing in-gel or in-solution digestion protocols.