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Selecting the Appropriate Laboratory Equipment: The Coomassie (Bradford) Protein Assay Performed Manually and Automated

Gilson, Inc.
2 Aug 2012

This application note discusses the determination of protein concentration in a solution using the Coomassie (Bradford) Protein Assay spectroscopic technique. The Bradford Assay was performed using a manual method (PIPETMAN M Multichannel and PIPETMAN L) and automated methods (GX-274 ASPEC and GX-241 Liquid Handler).

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PIPETMAN® L

Gilson, Inc.

PIPETMAN L increases your comfort for daily pipetting. Robust, Comfortable and Lightweight: With the PIPETMAN® L, you can now enjoy an extremely lightweight pipette that has been designed to fit naturally in your hand. Its new shape ensures a perfect fit while the new hook reduces tensions while holding it. Extremely Low Pipetting Forces: PIPETMAN® L offers an enhanced new design resulting in extremely low pipetting forces minimizing the onset of fatigue. A new piston assembly has been developed to drastically improve the pipetting and purge forces. A new shape and new mechanism for the tip ejection system dramatically reduce the tip ejection forces by up to 50% over the PIPETMAN®Neo. Accurate Pipetting: PIPETMAN® L range guarantees the best results with better performance than ISO specifications. Gilson Patented Volume Locking System: The locking system guarantees the accuracy of the delivered volume while the GLP-compliant thumbwheel ensures a comfortable and reliable means of changing the volume. 2D Identification Code for Easy Traceability: This tracking feature includes the product reference, date of manufacture, the serial number and the nominal volume. PIPETMAN® L is available in 8 single channel models covering a volume range from 0.2 μL to 10 mL.

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MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Pipettes and DispensersPipettes and dispensers are essential lab equipment used to dispense measured volumes of liquid accurately. Explore a range of manual and electronic pipettes, including single and multichannel, serological and volumetric pipettes, as well as a range of pipette tips. Bottle-top dispensers are also available for simple and fast solvent dispensing. Find the best pipettes & dispensers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Robotic WorkstationsRobotic workstations are automated platforms enabling the user to perform high-throughput, multi-application processes, from sample preparation tasks such as PCR set-up, DNA purification and NGS library preparation, to integrated sample prep and analysis workstations. Automation makes processes consistent, fast, precise and fully walk-away. Find the best robotic workstations in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bradford Protein Assay

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Selecting the Appropriate Laboratory Equipment: The Coomassie (Bradford) Protein Assay Performed Manually and Automated