ResourceLife Sciences
Screening for Inhibitors of BRD4 Bromodomain Using a Homogeneous Proximity Assay
14 Oct 2012The BET family of proteins, which contain tandem repeats of Bromodomains plus an Extra Terminal domain, play a role in bridging acetylation and transcriptional regulation of diverse cellular processes such as inflammatory gene expression, mitosis and viral/host interactions. Several small molecule inhibitors have been identified that disrupt bromodomain/acetylated lysine interactions. In this application note from BioTek, the automation of a homogeneous TR-FRET assay method suitable for high throughput screening and rapid characterization of inhibitors of bromodomain/acetylated peptide interactions is described.
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Agilent BioTek MultiFlo FX Multimode Dispenser
Agilent TechnologiesMultiFlo FX can dispense up to 4 independent reagents into 6- to 1536-well microplates.
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