Screening for Cell Adhesion Properties

In this application note, NanoInk’s desktop nanolithography platform, the NLP 2000 System, was used to create sub-cellular scaled arrays of multiple extracellular matrix (ECM) proteins on a glass slide. The multiplexed arrays were used to determine the cell binding efficiencies of various cell types, and binding efficiency data was used to identify the optimal ECM and array geometry for each cell type being studied. Using this technique, it was possible to establish a cell’s adhesion propensities for multiple ECM proteins over the course of a single screening experiment.