Scaling from Analytical to Preparative Chiral Chromatography While Balancing Purity, Yield, and Throughput under HPLC and SFC Conditions

The work presented in this application will address the relationship between both normal phase and SFC chiral methodologies at the analytical and preparative scale. The impact on resolution at both scales due to flow rates will be evaluated and compared between SFC and normal phase. The effect of preparative column hardware technology along with resulting purity and throughput from related SFC and normal phase purification methodologies will also be evaluated. Axia™ preparative column technology along with Axia specialized hardware shows higher performance than traditionally packed standard hardware preparative columns. The Axia packing technology is compatible with both SFC and HPLC conditions. In this application, we will demonstrate how the Axia packed columns with the Lux® Cellulose-1 polysaccharide-based chiral stationary phase can be a tool to increase throughput for purification of chiral compounds.