Sapphire FL imaging of multiplex fluorescent immunohistochemistry slides

4 Jul 2024

Immunohistochemistry (IHC) is a widely used technique for identifying antigens in tissue specimens on slides. While IHC slides are typically imaged using a microscope, fluorescent scanners like the Sapphire™ FL Biomolecular Imager are useful for rapid triage and whole-slide documentation. Azure Biosystems demonstrates the use of the Sapphire FL Biomolecular Imager to image a multiplex fluorescent IHC experiment. Using standard optical modules, the Sapphire FL is used to detect two antigens in a tissue section of mouse lung. Discover how using the Sapphire FL for rapid triage allows researchers to quickly select the best slides for microscopic analysis. In addition, images of whole slides (compared to small sections of slides captured by microscopes) can supplement microscope images and assist in documentation.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.ImmunohistochemistryImmunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples by using antibodies that bind to target antigens. IHC is widely applied in pathology, immunology, hematology and cancer diagnostics. Explore IHC tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Fluorescence Scanning
Sapphire FL imaging of multiplex fluorescent immunohistochemistry slides