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Sample quality assessment of nucleic acids with Agilent Fragment Analyzer systems

Agilent Technologies

Assessing sample quality with Agilent Fragment Analyzer for nucleic acids

5 Apr 2023

This application note discusses how the Agilent Fragment Analyzer systems can be used for nucleic acid sample assessment in various fields such as pharmaceuticals, genetic testing labs, and clinical research. The Fragment Analyzer systems promise reliable and accurate nucleic acid sample assessment, and a range of application kits are available for different types of samples. The systems can be used in various workflows such as genomic DNA, next-generation sequencing library preparation, and mRNA analysis, and this application note outlines the advantages of sample QC and how it can be incorporated into genomic workflows.

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Fragment Analyzer Systems

Agilent Technologies

The Agilent Fragment Analyzer systems are fluorescence-based capillary electrophoresis instruments for the accurate sizing, quantification, and qualification of nucleic acids (DNA and RNA).

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Capillary ElectrophoresisCapillary electrophoresis (CE) is used to separate molecules based on charge, size and hydrophobicity. Pressure, voltage or a vacuum is used to introduce the sample to fused silica capillaries. Fluorescence, UV/Vis, UV or diode array detectors are used to visualize components. Types of CE systems include capillary electrochromatography (CEC), capillary zone electrophoresis and capillary gel electrophoresis. Find the best capillary electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.DNA QuantificationDNA quantification is the process of measuring the amount of DNA in a sample. It is vital in genomics, molecular biology, and biotechnology, helping to ensure accurate experiments and data analysis. Explore DNA quantification tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Sample ProcessingSample processing is required to prepare samples prior to analysis. Technologies used for sample processing include homogenizers, grinders, mills and mixers.

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Sample quality assessment of nucleic acids with Agilent Fragment Analyzer systems