Same-section spatial multiomic analyses for investigating the dynamics of the tumor microenvironment

The recent increase in image-based, spatially-resolved technologies enables researchers to profile the tumor microenvironment (TME) by capturing gene expression profiles within tissue sections. However, a significant limitation of these technologies is the lack of ability to resolve protein and RNA information in the same section, as well as conveniently analyze multimodal data sets.

Here, we report a spatial RNA detection method, RNAsky, using Miltenyi Biotec’s MACSima™ Platform as an automated, multiomic approach. The MACSima^™ Imaging Cyclic Staining technology provides the capability to image RNA transcripts and protein markers in a single tissue section, enabling in-depth, single-cell resolution analyses of spatial architecture and identification of key immuno-oncology markers. Download this poster to learn more.