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Robust, reproducible, and easy-to-implement plasma protein profiling workflow using high-flow UHPLC/MS/MS without depletion

Thermo Fisher Scientific
10 Nov 2023

The concentration levels of salt, drugs, metabolites, and proteins contained inside plasma span 12 orders of magnitude. Knowing the function and composition of plasma is vital to our health as plasma is a major carrier for biomarkers of disease. It functions to transport nutrients, hormones, and cells, and plays an active role in innate immune response and clotting. In a study of protein-based bio markers, 20,000 proteins are reported in plasma. However, the top 20 constitute 98% of the mass of all proteins, making identifying and quantifying low concentration proteins of interest difficult as a routine practice.

To facilitate the identification and quantification of plasma biomarkers for clinical research, Thermo Fisher Scientific developed a highly robust liquid chromatography mass spectrometry-based protein profiling workflow for the analysis of undepleted plasma. In this application note, Thermo Fisher Scientific discusses the durability of the chromatography system to ensure its capability of handling a large volume injection of plasma samples, hundreds of injections before deep cleaning, and the minimum of carryover.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. BiosensorsBiosensors are devices used to detect an analyte using biological molecules specific to the analyte coupled to a detector. Biosensor instruments may be photometric, typically using surface plasma resonance (SPR), electrochemical or QCM (quartz crystal microbalance) biosensors. Biosensors should be selective, portable, robust and sensitive and have a fast response time. Systems may be manual or automated and usually have associated software.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :

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Robust, reproducible, and easy-to-implement plasma protein profiling workflow using high-flow UHPLC/MS/MS without depletion