Robust characterization of heterogeneity in CAR T cell cytotoxicity

The detection and analysis of single cells are critical in advancing CAR T cell therapy and understanding its mechanisms of action, such as cytotoxicity. Traditional bulk assays fall short of revealing cellular heterogeneity, which limits their effectiveness in evaluating therapeutic potency. In this application note, the Xdrop single-cell assay from Samplix addresses this challenge by providing high-resolution insights into CAR T cell-mediated cytotoxicity. With the ability to quantify granzyme B secretion and cell-killing activity at the single-cell level, Xdrop offers precise characterization of CAR T cells' functional diversity, enabling automated and detailed analysis of immune cell therapies.