Robust and reproducible neurofilament light quantitation in serum and plasma samples using Ella

20 Nov 2020

Neurofilament light (NF-L) has emerged as a biomarker for diagnosing and monitoring the progression of a variety of neuropathies such as Huntington’s disease, amyotrophic lateral sclerosis (ALS), Alzheimer’s disease, and multiple sclerosis. The Simple Plex NF-L Assay on Ella delivers the sensitivity required to measure NF-L with a high level of reproducibility in serum, plasma and CSF sample types. This application note demonstrates the Simple Plex NF-L Assay’s ability to distinguish between normal and disease state samples.

Ella Automated Immunoassay System

Bio-Techne

Ella is an automated ELISA platform that fits right on the benchtop of any laboratory. Ella simplifies multiplexing and presents consistent biomarker detection in less than 90 minutes, using only 25 µL of sample, and generating triplicate data, offering more flexibility than other instruments within the immunoassay space. 

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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Multiple SclerosisAlzheimers ResearchAlzheimer�s disease is a neurodegenerative condition characterized by progressive memory loss and cognitive decline. Research focuses on understanding the molecular and cellular mechanisms of the disease, as well as developing effective treatments and diagnostic tools. Browse our peer-reviewed product directory to find the best Alzheimer�s research products, compare options, check reviews, and get pricing directly from manufacturers.Huntingtons DiseaseNeurodegenerationNeurodegeneration refers to the progressive deterioration of the structure and function of the nervous system, often seen in diseases like Alzheimer's and Parkinson's. Research focuses on early detection and potential therapies. Explore neurodegeneration research tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Biomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.
Robust and reproducible neurofilament light quantitation in serum and plasma samples using Ella