ResourceLife Sciences
RNA Quality Score (RQS) Calculation and Correlation to RIN
26 Oct 2011Caliper Life Sciences’ LabChip GX system analyzes RNA by electrophoretic separation on microfluidic sipper chips. RNA molecules are separated and subsequently detected via laser induced fluorescence. In this application note, it is demonstrated that the system can, amongst other advantages, provide single number rating of RNA quality that can qualify RNA samples prior to use in gene expression studies.
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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.