RNA Isolation from 3D Microtissues Using the Promega ReliaPrep™ RNA Tissue Miniprep System

16 Dec 2015

This application note details a protocol to isolate total RNA from as little as 1 microtissue (~ 1000 cells) using the ReliaPrep™ RNA Tissue Miniprep System. Isolation of high quality total RNA from cultured cells is important for various analyses in cell-based drug discovery and toxicity. Analytical applications such as quantitative real time PCR or microarray analysis depend on robust RNA isolation procedures.

Related products

Request Quote for All Products

3D InSight™ Human Liver Microtissues
InSphero
Discover the 3D InSight™ Human Liver Microtissues: the gold standard for Hepatotoxic screening. Pharma-validated co-culture models offer 2x higher DILI prediction sensitivity than 2D hepatocytes. Features pooled PHH from male/female donors co-cultured with Kupffer cells and LECs, 28-day viability, in 96-well format. Includes 50mL 3D InSight™ Liver Maintenance Medium. Check the bi-weekly production schedule online.
(0)
QuantiFluor™ RNA System
Promega Corp.
The QuantiFluor™ RNA System contains a fluorescent RNA-binding dye that enables sensitive quantitation of small amounts of RNA in solution. Detecting and quantitating small amounts of RNA is a very important step used in many biological applications, particularly in molecular biology techniques. These include measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation and reverse transcription PCR. Features - Benefits Increase your Sensitivity: Significantly increased sensitivity over absorbance at 260nm (NanoDrop®) for those samples that are low in concentration. Save Precious Sample for Downstream Assays: Less template RNA required than spectrophotometry. Setup Quickly and Easily: System includes all the necessary reagents to quickly set up and quantitate RNA. Experience Flexible Instrument Compatibility: Sets up easily on both the QuantiFluor™ and GloMax®-Multi Instrument lines. This system also can be used on any fluorescent instrument with appropriate optical channels. Remain Cost-Effective: Value priced for those customers who are cost-conscious and budget-constrained. Applications RNA quantitation. Notes Each QuantiFluor™ RNA System contains sufficient volume to perform 200 assays at 2ml or 2,000 assays at 200μl.
(5)
ReliaPrep™ RNA Miniprep Systems
Promega Corp.
The ReliaPrep™ RNA Miniprep Systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes. The proprietary column/binding matrix can efficiently capture RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15µl). Using this membrane-based purification system, from 100 to 5 × 106 cultured cells or from 0.25 to 20mg of tissue can be processed per purification. ReliaPrep™ RNA Miniprep Systems Features & Benefits: Be Efficient - Allows use of hard-to-obtain precious samples. Have Confidence - Provides maximum sensitivity for downstream assays without worry of inhibition when measuring low-copy-number targets. Save Effort - No need to further concentrate samples for use. Save Time - Rapid protocol and provided DNase reagents streamline laboratory processes.
(2)
GoTaq® 1-Step RT-qPCR System
Promega Corp.
Brighter signal for earlier quantification of low and high copy RNA targets in a convenient 1-Step RT-qPCR format GoTaq® 1-Step RT-qPCR System is a reagent system that gives a brighter signal for earlier quantification of low and high copy RNA targets, using a one-step reverse transcription-quantitative PCR (RT-qPCR) protocol in a single tube format. The system incorporates BRYT Green® Fluorescent Dye, a novel dye that exhibits enhanced fluorescence when bound to dsDNA, and optimized buffer formulations to improve data accuracy and sensitivity of low expressing targets or targets present at low amounts. GoTaq 1-Step RT-qPCR System is compatible with all real-time PCR instruments in both fast and standard cycling conditions. The optimized formulations of the GoTaq 1-Step RT-qPCR System components have been engineered to enable simple reaction setup and provide consistently efficient, sensitive, broad range and robust performance in all standard or fast instrument platforms. The components and protocol allow cDNA synthesis using the GoScript™ Reverse Transcription System and quantification using the GoTaq® qPCR Master Mix. GoTaq 1-Step RT-qPCR System can be used with any real-time instrument capable of detecting SYBR® Green I or FAM™ dye and contains a low level of CXR Reference Dye. A separate tube of CXR Reference Dye is included for use with instruments that require a higher level of reference dye. .
(0)