ResourceSeparations
Ribose and Xylose: Retention and separation of simple sugars
23 Mar 2022Sugars can be difficult to analyze with HPLC due to their polarity. Columns with amine ligands are often used for retention of simple sugars like Ribose and Xylose, but they have a number of drawbacks.
In this application note, MicroSolv Technology Corporation demonstrates that the Cogent Amide Column avoids these problem because its ligand is less chemically reactive than an amine, while still obtaining good retention and separation of the two sugar analytes.
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Cogent Amide Hydride™
MicroSolv Technology Corp.Cogent Amide Hydride™ HPLC Columns are manufactured with TYPE-C™ silica (silica hydride) and have an ethyl amide functional group bonded to the silica particle with direct, silicone-carbon bonds. The manufacturing of silica makes these columns extremely stable.
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