Reverse Phase Protein Arrays for Cell Signaling: Using the InnoScan 710-IR Scanner Improves Sensitivity and Dynamic Range
16 May 2013Reverse Phase Protein Arrays (RPPAs) are increasingly used to study cell signaling as they are highly multiplexed and allow precise quantification of protein of interests in biological samples. The InnoScan 710-IR scanner combines infrared detection with high sensitivity and quality images. This application note compares the performance of the InnoScan 710-IR to its main competitor infrared scanner. It is shown that the InnoScan 710-IR improves the signal to noise ratio by a factor of up to 2.7 especially for low concentrations of arrayed cell lysates and extends the dynamic range compared to its competitor.
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Advanced Infrared 2-colour Microarray Scanner: InnoScan 710IR
InnopsysUnmatched performance for protein assays.Specifically designed to enhance detection in nitrocellulose protein array applications, the InnoScan 710IR near infrared (NIR) fluorescence scanner from Innopsys has on-board 670 nm and 785 nm excitation lasers to optimize performance by reducing the inherent background from the nitrocellulose. No scanner comes close for optical quality and speed of operation. Full compatibility with traceability standards is guaranteed, with a validation slide and associated Mapix software provided.Eliminating one of the most common concerns, the InnoScan 710IR is insensitive to substrate distortion. The real-time autofocus system makes the entire reading area completely uniform.Sensitivity and signal-to-noise ratio are two of the outstanding characteristics upon which the reputations of InnoScan scanners have been built. Outstanding photometric properties, combined with unique data manipulation software, ensure the most advanced analyses possible on the market for this class of scanner.Features: 670 nm and 785 nm excitation lasers for enhanced IR performance Simple to understand and operate – concentrate on the interpretation High precision - maximum resolution of 3 µm/pixel Under 4 minutes reading time combined with 10µm resolution Unbeatable scanning speed (10 to 35 lines/sec) Barcode identification for reliable traceability Portable, compact design; can be shared by labs Attractive price