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Revealing cellular metabolic phenotype and function using Agilent XF substrate oxidation stress tests

Agilent Technologies
31 Jul 2023

In this application note, Agilent Technologies showcases its Seahorse XF technology, which enables real-time measurement of energy metabolism in live cells, offering vital functional insights related to cellular health and fitness. The Agilent Seahorse XF Substrate Oxidation stress test provides essential metrics for investigating specific mitochondrial substrates that impact cellular phenotype and function. The study includes an assay strategy overview, data examples, and guidance on applying these methods in cancer and immunological cell metabolism research for drug discovery.

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Seahorse XF Palmitate Oxidation Stress Test Kit and FAO Substrate

Agilent Technologies

The Agilent Seahorse XF Palmitate Oxidation Stress Test Kit provides validated reagents to measure long chain fatty acid oxidation (FAO) via assessing changes in oxygen consumption (OCR) in intact cells. The kit includes the XF Palmitate-BSA FAO Substrate, L-carnitine, etomoxir, as well as oligomycin, FCCP, and rotenone/antimycin A to determine the intrinsic rate and capacity of a cell to oxidize palmitate in the absence or limitation of other exogenous substrates. This kit is best applied when

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