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Resolution Of Normal Phase Flash Chromatography Improved by Dry Samples

Teledyne Labs

Resolution Of Normal Phase Flash Chromatography Improved by Dry Samples

20 Jul 2015

This application note demonstrates that preparing dry samples using RediSep® solid load sample cartridges improves peak resolution over liquid injection techniques. When the compound and reaction by-products are over-diluted or dissolved in too polar a dissolving solvent, the compound peak broadens which leads to loss of resolution. The effects of band broadening and overlapping peaks were gauged using dual UV detection/monitoring.

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CombiFlash® Torrent

Teledyne Labs

Get the ease of use and reliability provided by CombiFlash and RediSep for Development Scale purification with the CombiFlash Torrent Purification System from ISCO. One liter per minute flow rates rapidly purify up to 300 grams of sample. The 100 psi pumps accurately deliver a reproducible binary gradient. The CombiFlash Torrent system is designed to be flexible for a variety of large-scale purification needs, with various methods of sample introduction and collection.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Flash ChromatographyFlash chromatography is a fast-preparative column chromatography technique used for separation and purification of liquid analytes. Flash chromatography systems use small silica gel particles for separation and a pressurized gas for driving the solvent through the column or pre-packed cartridge. Find the best flash chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLC Method DevelopmentIt is important to develop and validate HPLC methods in order to identify, quantify or purify compounds of interest in the most efficient way. Effective method development ensures optimization, quality control and cost effectiveness.UVUltraviolet (UV) light is used in a variety of applications, including disinfection, spectrophotometry, and molecular biology. UV light can damage DNA, making it a powerful tool in sterilization and in studies of mutagenesis and gene repair. Explore UV-related products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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